Ordered chimerogenesis applied to CYP2B P450 enzymes
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- 作者：Thomas Lautiera ; b ; c ; 1 ; Philippe Urbana ; b ; c ; 1 ; Jacqueline Loeperd ; e ; Laetitia Jezequelf ; Denis Pompona ; b ; c ; Gilles Truana ; b ; c ; gilles.truan@insa-toulouse.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：P450 ; CYP2B6 ; CYP2B11 ; Chimera ; Cyclophosphamide ; Channel
- 刊名：Biochimica et Biophysica Acta (BBA) - General Subjects
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：1860
- 期：7
- 页码：1395-1403
- 全文大小：1382 K
文摘
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A series of fifteen 2B6/2B11 chimeras have been expressed in yeast.
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Some chimeras present an altered affinity for cyclophosphamide.
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Within the F/G cassette, the F′/G′ loop is shown to control affinity for CPA.
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The affinities for EFC of chimera enzymes are mostly unaffected.