Validation of IgG, IgM multiplex plasmonic gold platform in French clinical cohorts for the serodiagnosis and follow-up of Toxoplasma gondii infection

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• 作者：Christelle Pomares^a ; ^b ; ^c ; ^d ; ¹ ; ^{pomares.c@chu-nice.fr} ; Bo Zhang^e ; ¹ ; ^{bo.zhang@nirmidas.com} ; Shylaja Arulkumar^e ; Geraldine Gonfrier^f ; Pierre Marty^c ; ^d ; Su Zhao^e ; Steven Cheng^e ; Meijie Tang^e ; Hongjie Dai^g ; Jose G. Montoya^a ; ^b
• 关键词：Toxoplasma gondii ; Plasmonic gold pGOLD ; Multiplex serology ; Seroconversion ; Newborns ; Adults
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：87
• 期：3
• 页码：213-218
• 全文大小：230 K
• 卷排序：87

文摘

We report the use of the multiplexed T. gondii IgG, IgM test on plasmonic gold (pGOLD) platform in the setting of T. gondii infection by analyzing 244 sera from Nice, France (seroconversion, chronically infected, non-infected and newborns serum samples). Results were compared with commercial tests for the detection of IgG and IgM and their overall clinical final interpretation of a complete serological profile. The IgG and IgM test results on the platform were in agreement in, respectively, 95% and 93% with the commercial kits. When comparing with the overall clinical interpretation of the serological profile, the agreement reached 99.5% and 97.7% for IgG and IgM, respectively. This innovative pGOLD platform allows detection of both IgG and IgM simultaneously with only ~1 microliter of serum. The multiplexed IgG/IgM test on pGOLD platform is a strong candidate for its use in the massive screening programs for toxoplasmosis during pregnancy.