Identification of ER membrane targeting signal of kinectin

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• 作者：Erika Abe ; Satoshi Okawa ; Masashiro Sugawara ; Sumio Watanabe ; Itaru Toyoshima
• 关键词：Kinectin ; Endoplasmic reticulum ; Transmembrane domain
• 刊名：Neuroscience Letters
• 出版年：2007
• 出版时间：21 February 2007
• 年：2007
• 卷：413
• 期：3
• 页码：238-240
• 全文大小：733 K

文摘

Kinectin has been identified as a kinesin receptor on endoplasmic reticulum (ER). The ER membrane binding domain of kinectin is still obscure and is thought to require a half of the molecule. To determine the ER insertion site, we produced several constructs around N-terminus of kinectin connected with green fluorescent protein (GFP) and visualized the distribution in Cos-7 cells. The fragment of residues 7–29 appeared in the reticular pattern exactly colocalized with the ER marker but did not remain for a long time. On the other hand, residues 1–106 maintained a reticular pattern for more than seven days. These results indicate that residues 7–29 of kinectin are sufficient for targeting to the ER membrane but insufficient for remaining on the ER.