Whole genome and normalized mRNA sequencing reveal genetic status of TK6, WTK1, and NH32 human B-lymphoblastoid cell lines
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- 作者：Javier Revolloa ; Dayton M. Petibonea ; Dayton.petibone@fda.hhs.gov" class="auth_mail" title="E-mail the corresponding author ; Page McKinziea ; Bridgett Knoxb ; Suzanne M. Morrisa ; 1 ; Baitang Ningb ; Vasily N. Dobrovolskya
- 关键词：Next generation sequencing (NGS) ; p53 ; FTH1 ; TPMT*3A ; NQO1*2 ; Human B-lymphoblastoid cells
- 刊名：Mutation Research/Genetic Toxicology and Environmental Mutagenesis
- 出版年：2016
- 出版时间：1 January 2016
- 年：2016
- 卷：795
- 期：Complete
- 页码：60-69
- 全文大小：1160 K
文摘
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Predicted genes, SNPs and mutations in TK6 are similar to the human population.
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TK6 (p53wt), WTK1(p53mut) and NH32(p53KO) transcriptomes have not diverged greatly.
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NGS identified a mutation in the FTH1 in TK6 that impairs iron metabolism.
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SNPs in the TPMT (TPMT*3A SNP), and NQO1 (NQO1*2 SNP) genes impair drug metabolism.
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The FTH1 mutation, TPMT*3A and NQO1 SNPs have toxicological and clinical relevance.