Effect of gamma radiation on the identification of bacterial pathogens by MALDI-TOF MS

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• 作者：Dobryan M. Tracz ; Stuart J. McCorrister ; Garrett R. Westmacott ; Cindi R. Corbett ; ^{Cindi.Corbett@phac-aspc.gc.ca}
• 关键词：MALDI-TOF ; matrix-assisted laser desorption/ionization time-of-flight ; MS ; mass spectrometry ; NML ; National Microbiology Laboratory (Canada) ; BSL-2 ; biosafety level 2 ; BSL-3 ; biosafety level 3 ; ELISA ; enzyme-linked immunosorbent assay
• 刊名：Journal of Microbiological Methods
• 出版年：2013
• 出版时间：15 February, 2013
• 年：2013
• 卷：92
• 期：2
• 页码：132-134
• 全文大小：298 K

文摘

MALDI-TOF MS is a well-established method for rapid identification of bacteria; however there are no reports to date on its performance with gamma-irradiated samples typically used in BSL-3 laboratories for sample inactivation. In this report we demonstrate that gamma-irradiated bacteria can be accurately identified by MALDI-TOF MS in most cases, but a decrease in identification scores is observed.