Toxicity evaluation of new antifouling compounds using suspension-cultured fish cells

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• 作者：Okamura ; H. ; Watanabe ; T. ; Aoyama ; I. ; Hasobe ; M.
• 关键词：Antifouling compound ; Fish ; Rainbow trout ; Suspension-cultured cells
• 刊名：Chemosphere
• 出版年：2002
• 出版时间：February, 2002
• 年：2002
• 卷：46
• 期：7
• 页码：945-951
• 全文大小：138 K

文摘

A simple, rapid toxicity test was developed using the suspension-cultured fish cell line CHSE-sp derived from chinook salmon Oncorhynchus tshawytscha embryos in order to assess the toxicity of new marine antifouling compounds. The compounds tested were copper pyrithione, Diuron, Irgarol 1051, KH101, Sea-Nine 211, and zinc pyrithione, all of which have been nominated in Japan as possible replacements for organotin compounds. The in vitro acute toxicity (24-h EC₅₀) of the six compounds to these fish cells was evaluated using the dye Alamar Blue™ to determine cell viability, and then correlated with the results of in vivo chronic toxicities (28-day LC₅₀) to juvenile rainbow trout Oncorhynchus mykiss. The suspension-cultured fish cells were found to be suitable for the screening of such chemicals before performing an in vivo test. The toxicities of the test compounds obtained from both tests, shown in decreasing order, were as follows: copper pyrithione>zinc pyrithione>KH101≥Sea-Nine 211>Diuron>Irgarol 1051. The herbicides Diuron and Irgarol 1051 showed the least toxicity, while the pyrithiones had the greatest toxicity.