Biodecolorization of Textile Dyes by Immobilized Enzymes in a Vertical Bioreactor System

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• 作者：Dede Heri Yuli Yanto^a ; ^b ; ^{dede@biomaterial.lipi.go.id" class="auth_mail} ; Sanro Tachibana^c ; ^{tatibana@agr.ehime-u.ac.jp" class="auth_mail} ; Kazutaka Itoh^c ; ^{itoh@agr.ehime-u.ac.jp" class="auth_mail}
• 关键词：biodecolorization ; bioreactor ; immobilized enzymes ; Pestalotiopsis sp ; textile dyes ; Trametes versicolor
• 刊名：Procedia Environmental Sciences
• 出版年：2014
• 年：2014
• 卷：20
• 期：Complete
• 页码：235-244
• 全文大小：612 K

文摘

A capacity of immobilized enzymes from Trametes versicolor U97 or Pestalotiopsis sp. NG007 to decolorize three textile dyes was investigated in a vertical bioreactor system. Immobilization was conducted using a double layer of alginate bead (1.5% w/w) and crude enzymes. The effect of mediators: Tween 80, 1-hydroxybenzotriazol (HBT), or 2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO) was investigated on decolorization rate. The results showed that application of immobilized enzymes in a vertical bioreactor enhanced the decolorization efficiency of dyes in the order LB16>RRV9>RRN4, respectively. The decolorization rate was accelerated when a mediator TEMPO was used in immobilized enzyme of U97 or a Tween 80 was used in NG007. Reaction with glutaraldehyde (0.6%) for 4 h maintained the longevity and reusability of the beads. The study suggests that double layer immobilized enzyme in a bioreactor system has a terrific potential strategy for treating the textile dye effluents.