Origins of Programmable Nucleases for Genome Engineering
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- 作者：Srinivasan Chandrasegaran1 ; schandra@jhsph.edu" class="auth_mail" title="E-mail the corresponding author ; Dana Carroll2 ; dana@biochem.utah.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：DSB ; double-strand break ; ZFN ; zinc finger nuclease ; ZFP ; zinc finger protein ; HR ; homologous recombination ; TALEN ; transcription activator-like effector nuclease ; NHEJ ; non-homologous end joining ; HDR ; homology-directed repair ; R&ndash ; M ; restriction&ndash ; modification ; ZF ; zinc finger ; ZFA ; zinc finger transcription activator ; ZFR ; zinc finger transcription repressor ; ZFM ; zinc finger methylase ; OPEN ; oligomerized pool engineering ; CoDA ; context-dependent assembly ; RVD ; repeat variable di-resi
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：27 February 2016
- 年：2016
- 卷：428
- 期：5
- 页码：963-989
- 全文大小：2904 K
文摘
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Origins and development of programmable nucleases for genome engineering.
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ZFNs, TALENs and CRISPR-Cas9.
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Off-target effects.
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Applications of programmable nucleases in biology and medicine.
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Ethics and scientific issues concerning genome editing of human embryos.