TALENs-induced frameshifts in the myostatin (MSTN) coding sequence in medaka.
TALENs-mediated MSTN mutations could be transmitted to the next generation.
TALENs-mediated MSTN frame-shifted mutation induced the expression of myogenic regulator factors (MRFs).
The F2 MSTN−/− medaka showed significantly increased body length and weight compared to the wild type fish.
In the TALENs-mediated MSTN−/− fish, there is a failure to trigger an ISG response at least against this virus.