CRISPR/Cas9 genome editing of rubber producing dandelion Taraxacum kok-saghyz using Agrobacterium rhizogenes without selection
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- 作者：Brian Iaffaldano ; Yingxiao Zhang ; Katrina Cornish ; cornish.19@osu.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：TK ; Taraxacum kok-saghyz ; 1-FFT ; fructan ; fructan 1-fructosyltransferase ; ZFNs ; zinc finger nucleases ; TALENs ; transcription activator-like effector nucleases ; CRISPR ; clustered regularly interspaced short palindromic repeats ; Cas9 ; CRISPR associated protein 9 ; QTLs ; quantitative trait loci ; PEG ; polyethylene glycol ; 1/2 MS ; half-strength Murashige and Skoog medium ; sgRNA ; single guide RNA ; CTAB ; cetyl trimethyl ammonium bromide ; PCR ; polymerase chain reaction ; InDels ; insertions and deletions ; P
- 刊名：Industrial Crops & Products
- 出版年：2016
- 出版时间：30 October 2016
- 年：2016
- 卷：89
- 期：Complete
- 页码：356-362
- 全文大小：1331 K
文摘
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Agrobacterium rhizogenes can effectively deliver CRISPR/Cas9 to hairy roots, without selection.
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Hairy root populations were mutagenized at rates as high as 88.9%.
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Leaves regenerated from CRISPR/Cas9 treated hairy roots were mutagenized at rates as high as 80.0%.