(?-Epigallocatechin-3-gallate stimulates both AMP-activated protein kinase and nuclear factor-kappa B signaling pathways

详细信息    查看全文

• 作者：Kosuke Asano^a ; ^b ; Katsuhiro Takagi^a ; Ayumi Haneishi^a ; ^b ; Soichiro Nakamura^b ; Kazuya Yamada^a ; ^c ; ^{kazuya.yamada@matsu.ac.jp}
• 关键词：EGCG ; (&minus ; )-epigallocatechin-3-gallate ; SHARP ; enhancer of split- and hairy-related protein ; PEPCK ; phosphoenolpyruvate carboxykinase ; PI 3-K ; phosphoinositide 3-kinase ; aPKC¦Ë ; atypical protein kinase C lambda ; AMPK ; AMP-activated protein kinase ; NF-
• 刊名：Food Chemistry
• 出版年：2012
• 出版时间：15 September, 2012
• 年：2012
• 卷：134
• 期：2
• 页码：783-788
• 全文大小：385 K

文摘

We previously reported that (?-epigallocatechin-3-gallate (EGCG) increased the level of SHARP-1 mRNA via a phosphoinositide 3-kinase/atypical protein kinase C lambda signaling pathway in rat H4IIE hepatoma cells. In the present study, we investigated other signaling pathway(s). Treating with either compound-C, BAY11-7082, or both, partially blocked the up-regulation of the SHARP-1 gene by EGCG. This suggests that AMP-activated protein kinase (AMPK)- and nuclear factor-kappa B (NF-¦ÊB)-signaling pathways were additively involved in the induction mediated by EGCG. Indeed, an AMPK activator induced a level of SHARP-1 mRNA. Although actinomycin D partially blocked the EGCG-induction of that SHARP-1 mRNA level, the nucleotide sequence between ?501 and ? in the rat SHARP-1 gene did not positively respond to EGCG and NF-¦ÊB, respectively. Thus, we conclude that EGCG stimulates multiple signaling pathways in the SHARP-1 gene expression at the transcriptional and post-transcriptional levels and that there is no regulatory region susceptible to EGCG and NF-¦ÊB in the examined region.