- 作者：Hiroaki Hane ; Yoshinao Muro ; ymuro@med.nagoya-u.ac.jp" class="auth_mail ; Kanako Watanabe ; Yasushi Ogawa ; Kazumitsu Sugiura ; Masashi Akiyama
- 关键词：ARS ; aminoacyl transfer RNA synthetase ; ACTD ; autoimmune connective tissue disease ; MDA5 ; melanoma differentiation-associated protein 5 ; ILD ; interstitial lung disease ; TIF1 ; transcriptional intermediary factor 1 ; IIM ; idiopathic inflammatory myopathy ; MSA ; myositis-specific autoantibody ; MAA ; myositis-associated autoantibody ; DM ; dermatomyositis ; PM ; polymyositis ; CADM ; clinically amyopathic dermatomyositis ; SLE ; systemic lupus erythematosus ; lSSc ; limited cutaneous systemic sclerosis ; dSSc ; di
- 刊名：Clinica Chimica Acta
- 出版年：20 April, 2014
- 年：2014
- 卷：431
- 期：Complete
- 页码：9-14
- 全文大小：585 K
Background
The aminoacyl transfer RNA synthetases (ARSs) are a group of enzymes that charge amino acids to the cognate transfer RNA during the translation process. Previous reports demonstrated autoantibodies to 8 different ARS. Although anti-glycyl-tRNA synthetase antibodies (anti-EJ) are mainly found in patients with inflammatory myopathy, information on their clinical significances is limited, partly due to a lack of commercially available tests.Methods
We developed an ELISA and immunoprecipitation method by using recombinant EJ protein to detect the anti-EJ of 453 patients with various autoimmune connective tissue diseases (ACTDs). We also studied the influence of 3 cytokines鈥擨L-1尾, IFN-纬 and IFN-伪鈥攐n the level of EJ mRNA and protein expressed by human fetal lung fibroblasts.
Results
Five patients were positive for anti-EJ. Although 3 of these patients had dermatomyositis/polymyositis, the other 2 patients did not have myositis. The three patients with high levels of anti-EJ antibodies in ELISA were complicated with interstitial lung disease. There was no significant change in the level of EJ protein expressed by human fetal lung fibroblasts stimulated by the cytokines.
Conclusion
We developed an ELISA to detect anti-EJ by using recombinant protein. This easy-to-use ELISA could help clarify the clinical significance of anti-EJ in ACTDs.