In vitro transport assays of rufinamide, pregabalin, and zonisamide by human P-glycoprotein

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• 作者：Pui Shan Chan^a ; Chunbo Zhang^a ; ¹ ; Zhong Zuo^a ; Patrick Kwan^b ; ^c ; Larry Baum^a ; ^{lwbaum@cuhk.edu.hk" class="auth_mail}
• 关键词：Pgp ; ABCB1 ; MDR1 ; Rufinamide ; Zonisamide ; Pregabalin
• 刊名：Epilepsy Research
• 出版年：March, 2014
• 年：2014
• 卷：108
• 期：3
• 页码：359-366
• 全文大小：2132 K

文摘

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Summary

Objective

Epilepsy is resistant to treatment with antiepileptic drugs (AEDs) in about one third of epilepsy patients. AED export by P-glycoprotein (Pgp) overexpressed in the blood-brain barrier may contribute to AED resistance. The Pgp transport status of many of the recently approved AEDs remains unknown. We investigated whether several new AEDs - zonisamide (ZNS), pregabalin (PGB), and rufinamide (RFM) - are human Pgp substrates.

Methods

MDCKII and LLC-PK1 cells transfected with the human MDR1 gene, which encodes the Pgp protein, were used in concentration equilibrium transport assays (CETA) to determine the substrate status of ZNS, PGB, and RFM. For each drug, an equal concentration was added to apical and basal chambers, and the concentration in both chambers was measured up to 4 h.

Results

RFM, ZNS, and PGB were not transported by MDR1-transfected cells from basolateral to apical sides in CETA.

Conclusions

ZNS, RFM, and PGB are not substrates of human Pgp. These data suggest that resistance to these drugs may not be attributed to increased Pgp activity in resistant patients.