Inhibition of versican expression by siRNA facilitates tropoelastin synthesis and elastic fiber formation by human SK-LMS-1 leiomyosarcoma smooth muscle cells in vitro and in vivo
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- 作者：Paul A. Keirea ; b ; Steven L. Bresslera ; Eileen R. Mulvihillb ; Barry C. Starcherc ; Inkyung Kanga ; Thomas N. Wighta ; b ; twight@benaroyaresearch.org" class="auth_mail" title="E-mail the corresponding author
- 关键词：ECM ; extracellular matrix ; α-GAG ; α-glycosaminoglycan ; β-GAG ; β-glycosaminoglycan ; CS ; chondroitin sulfate ; SMCs ; smooth muscle cells ; LAM ; lymphangioleiomyomatosis ; COPD ; chronic obstructive pulmonary disease ; LMS ; leiomyosarcoma ; IHC ; immunohistochemistry ; LOX ; lysyl oxidase ; qRT-PCR ; quantitative real time-polymerase chain reaction ; WT ; LMS/wild type ; non-transfected wild type leiomyosarcoma cells ; EV ; LMS/empty vector ; leiomyosarcoma cells transfected with empty vector plasmid ; Scr ; LMS/siRN
- 刊名：Matrix Biology
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：50
- 期：Complete
- 页码：67-81
- 全文大小：1228 K
文摘
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Downregulation of versican in leiomyosarcoma cells leads to elastic fiber formation.
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Microarray analyses identified an increase in ECM- and elastin-related genes.
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Combining cDNA and TaqMan low density arrays was useful for discovery and validation.
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Results highlight a role for versican in regulating elastin fiber assembly in cancer.