Precursor supply strongly influences the yield of acetyl-CoA-derived products on sugars.
The native yeast pathway for cytosolic acetyl-CoA formation is ATP inefficient.
Pathways from glucose to acetyl-CoA have different ATP, NAD(P)H and carbon stoichiometries.
Optimal pathway configuration for acetyl-CoA generation is strongly product dependent.
Combinations of acetyl-CoA forming reactions should be considered in pathway design.