Double-stranded RNA-mediated interference of dumpy genes in Bursaphelenchus xylophilus by feeding on filamentous fungal transformants
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- 作者：Meng Wanga ; Diandong Wangb ; d ; Xi Zhanga ; Xu Wanga ; Wencui Liua ; Xiaomeng Houa ; Xiaoyin Huanga ; Bingyan Xieb ; xiebingyan@caas.cn" class="auth_mail" title="E-mail the corresponding author ; Xinyue Chenga ; c ; chengxy@bnu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pinewood nematode ; Bursaphelenchus xylophilus ; Silencing vector pDH-RH ; RNA interference (RNAi) feeding ; Dumpy gene ; Collagen ; Filamentous fungal transformant ; Agrobacterium tumefaciens-mediated transformation (ATMT)
- 刊名：International Journal for Parasitology
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：46
- 期：5-6
- 页码：351-360
- 全文大小：1857 K
文摘
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An effective silencing vector pDH-RH was constructed.
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RNAi interference feeding technology in Bursaphelenchus xylophilus was described.
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Morphological phenotypes of four B. xylophilus dumpy (dpy) genes were observed.
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Compensation and non-specific effects among Bx-dpy genes were found in B. xylophilus.