Factors affecting Agrobacterium-mediated transformation efficiency of kumquat seedling internodal stem segments
详细信息 查看全文
- 作者：Li Yanga ; 1 ; Wei Hua ; Yuming Xiea ; Yi Lib ; yi.li@uconn.edu" class="auth_mail" title="E-mail the corresponding author ; Ziniu Denga ; deng7009@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：AS ; Acetosyringone ; BAP ; 6-Benzyladenine ; CCM ; Co-cultivation medium ; Cef ; Cefotaxime ; GUS ; β-Glucuronidase ; IM ; Inducible medium ; Km ; Kanamycin ; KT ; Kinetin ; MS ; Murashige and Skoog 1962 ; NAA ; α-naphthaleneacetic acid ; nptⅡ ; Neomycin phosphotransferase Ⅱ ; Rif ; Rifampicin ; SRM ; Shoot regeneration and selection medium ; YEB ; Yeast extract broth medium
- 刊名：Scientia Horticulturae
- 出版年：2016
- 出版时间：19 September 2016
- 年：2016
- 卷：209
- 期：Complete
- 页码：105-112
- 全文大小：1414 K
文摘
- •
We performed a comprehensive and systematic study on kumquat to improve transformation efficiency.
- •
Kumquat seedlings as transformation explants needed an appropriate period of illumination.
- •
The combination of Agrobacterium OD600 0.5, infection 30 min and co-cultivation 3 days with sterile filter paper support obtained the highest GUS positive rate.
- •
The combination of pH 5.4, 100 μM of AS and at 25 °C in co-cultivation obtained the highest GUS positive rate.
- •
The transformation efficiency was influenced by different placement way of explants in co-cultivation period.