KSHV LANA and EBV LMP1 induce the expression of UCH-L1 following viral transformation

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• 作者：Gretchen L. Bentz ; Anjali Bheda-Malge ; Ling Wang ; Julia Shackelford ; Blossom Damania ; Joseph S. Pagano
• 关键词：KSHV ; LANA ; EBV ; LMP1 ; UCH-L1
• 刊名：Virology
• 出版年：5 January, 2014
• 年：2014
• 卷：448
• 期：Complete
• 页码：293-302
• 全文大小：912 K

文摘

Ubiquitin C-terminal Hydrolase L1 (UCH-L1) has oncogenic properties and is highly expressed during malignancies. We recently documented that Epstein-Barr virus (EBV) infection induces uch-l1 expression. Here we show that Kaposi's Sarcoma-associated herpesvirus (KSHV) infection induced UCH-L1 expression, via cooperation of KSHV Latency-Associated Nuclear Antigen (LANA) and RBP-J魏 and activation of the uch-l1 promoter. UCH-L1 expression was also increased in Primary Effusion Lymphoma (PEL) cells co-infected with KSHV and EBV compared with PEL cells infected only with KSHV, suggesting EBV augments the effect of LANA on uch-l1. EBV latent membrane protein 1 (LMP1) is one of the few EBV products expressed in PEL cells. Results showed that LMP1 was sufficient to induce uch-l1 expression, and co-expression of LMP1 and LANA had an additive effect on uch-l1 expression. These results indicate that viral latency products of both human 纬-herpesviruses contribute to uch-l1 expression, which may contribute to the progression of lymphoid malignancies.