The impact of 1,25-dihydroxyvitamin D3 on the expression of connective tissue growth factor and transforming growth factor-尾₁ in the myocardium of rats with diabetes

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• 作者：Lingling Wang^a ; ^{1506468624@qq.com" class="auth_mail} ; Tao Yuan^b ; ^{t75y@sina.com" class="auth_mail} ; Guoli Du^a ; ^{genemagic@126.com" class="auth_mail} ; Qiying Zhao^a ; ^{zhaoqy1980@163.com" class="auth_mail} ; Lijuan Ma^a ; ^{308950323@qq.com" class="auth_mail} ; Jun Zhu^a ; ^{zhujun6677@163.com" class="auth_mail}
• 关键词：1 ; 25-Dihydroxyvitamin D3 ; Diabetes mellitus ; Myocardial fibrosis ; Connective tissue growth factor ; Transforming growth factor-尾1
• 刊名：Diabetes Research and Clinical Practice
• 出版年：May, 2014
• 年：2014
• 卷：104
• 期：2
• 页码：226-233
• 全文大小：3441 K

文摘

Aims

To define whether 1,25-dihydroxyvitamin D3 (1,25-(OH)2 D3) can protect against myocardial fibrosis and to investigate its impact on the expression of connective tissue growth factor (CTGF) and transforming growth factor-尾₁ (TGF-尾₁) in the myocardium of rats with diabetes.

Methods

Male Sprague-Dawley rats were divided into normal (control), 1,25-(OH)2 D3 therapy, and diabetes groups. In the diabetes and 1,25-(OH)2 D3 therapy groups, type 2 diabetes models were established using a high-fat, high-sugar diet and streptozotocin. Rats in the 1,25-(OH)2 D3 therapy group were also treated with 1,25-(OH)2 D3. After 6 weeks, the body weight, cardiac weight, cardiac weight index, plasma glucose, lactic dehydrogenase (LDH) and creatine kinase (CK) were measured; morphological changes in the myocardium were observed using microscopy following hematoxylin-eosin and Masson staining. CTGF and TGF-尾₁ expressions in the myocardium were detected using immunohistochemistry staining and reverse transcription polymerase chain reaction.

Results

The body and cardiac weights of the rats in the diabetes and 1,25-(OH)2 D3 group were lower, but the cardiac weight index, plasma glucose, LDH and CK were higher compared with the control group (P < 0.05). The body weight and plasma glucose, LDH and CK were decreased in 1,25-(OH)2 D3 group compared with the diabetes group (P < 0.05). Pathological changes in the 1,25-(OH)2 D3 group were milder than the diabetes group. CTGF and TGF-尾₁ expression in the diabetes and 1,25-(OH)2 D3 groups were increased significantly, but in the 1,25-(OH)2 D3 group were significantly lower than diabetes group at the mRNA level.

Conclusion

1,25-(OH)2 D3 had a partially protective effect on myocardial fibrosis of diabetic rats, which might inhibit CTGF and TGF-尾₁ expression in the myocardial tissues.