- 作者：Reshef Tal ; David B. Seifer ; Aya Shohat-Tal ; Richard V. Grazi ; Henry E. Malter
- 关键词：Polycystic ovarian syndrome (PCOS) ; transforming growth factor-¦Â1 (TGF-¦Â1) ; soluble endoglin (sENG) ; controlled ovarian stimulation (COS) ; angiogenesis
- 刊名：Fertility and Sterility
- 出版年：2013
- 出版时间：August, 2013
- 年：2013
- 卷：100
- 期：2
- 页码：538-543
- 全文大小：249 K
Objective
To evaluate the relationship between transforming growth factor (TGF)-¦Â1 and its receptor, soluble endoglin (sENG), in the serum and follicular fluid of women with polycystic ovarian syndrome (PCOS) compared with that of non-PCOS normal ovulating women during controlled ovarian stimulation (COS).Design
Prospective case-control study.
Setting
Academic-affiliated assisted reproductive technology unit.
Patient(s)
Fourteen PCOS and 14 matched non-PCOS control women undergoing COS.
Intervention(s)
Serum was collected on day 3 (baseline), day of hCG, and day of retrieval. Follicular fluid (FF) was collected on day of oocyte retrieval. ELISA was performed to determine TGF-¦Â1 and sENG protein levels.
Main Outcome Measure(s)
Serum and FF levels of TGF-¦Â1 and sENG.
Result(s)
Serum TGF-¦Â1 did not change significantly during COS but was increased in PCOS compared with non-PCOS women on day 3 and days of hCG administration and oocyte retrieval. Serum sENG increased after hCG administration only in the non-PCOS control group. In addition, serum sENG was decreased in PCOS compared with non-PCOS control women on the days of hCG and retrieval. Accordingly, the bioavailability of TGF-¦Â1 (TGF-¦Â1/sENG ratio) was increased in women with PCOS compared with non-PCOS controls at all three time points. No differences in either factor were noted in FF between groups.
Conclusion(s)
The increased TGF-¦Â1 bioavailability in PCOS is not only due to increased TGF-¦Â1 levels but also to decreased levels of its receptor, sENG. These data suggest that increased TGF-¦Â1 bioavailability may contribute to the pathogenesis of PCOS and its increased risk for ovarian hyperstimulation.