Galactosylation of human erythropoietin produced by chimeric chickens expressing galactosyltransferase

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• 作者：Yusuke Kojima ; Jun Wakita ; Yujin Inayoshi^§ ; ; Ryosuke Suzuki ; Yuki Yamada ; Hidenori Kaneoka ; ^{kaneoka@nubio.nagoya-u.ac.jp" class="auth_mail} ; Ken-ichi Nishijima ; Shinji Iijima
• 关键词：Transgenic chicken ; Erythropoietin ; Galactose ; Galactosyltransferase ; N-Glycan
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：June 2014
• 年：2014
• 卷：117
• 期：6
• 页码：676-679
• 全文大小：482 K

文摘

Human erythropoietin produced in the egg white of chimeric chicken contains N-glycan with lower amounts of terminal galactose and sialic acid; therefore, the chicken galactosyltransferase gene was introduced together with the human erythropoietin gene by a retroviral vector. We found that erythropoietin accumulated in the egg white was partially galactosylated.