Production and characterization of recombinant human acid α-glucosidase in transgenic rice cell suspension culture
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- 作者：Jae-Wan Junga ; b ; 1 ; Nan-Sun Kima ; 1 ; Seon-Hui Janga ; Yun-Ji Shina ; Moon-Sik Yanga ; b ; mskyang@jbnu.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pompe disease ; Acid α-glucosidase (GAA) ; Rice α-amylase 3D (RAmy3D) promoter ; Agrobacterium tumefaciens ; Transgenic rice cell suspension culture
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：20 May 2016
- 年：2016
- 卷：226
- 期：Complete
- 页码：44-53
- 全文大小：2045 K
文摘
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We constructed transgenic rice cell suspension culture producing human GAA.
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The expression of rhGAA was determined by Northern and western blot analyses.
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The accumulated rhGAA protein in the culture medium was 37 mg/L.
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The rhGAA was purified using a Ni-NTA column.
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The acid alpha-glucosidase activity of hGAA produced in transgenic rice cells gave results similar to those of the enzyme produced by CHO cells.