Direct translational analysis of electrophoretically separated DNA using a cell-free protein synthesis system

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• 作者：Kyung-Ho Lee¹ ; Jin-Ho Kwon¹ ; Dong-Myung Kim ; ^{dmkim@cnu.ac.kr}
• 关键词：Cell-free protein synthesis ; Electrophoresis ; Translational analysis ; Screening
• 刊名：Journal of Industrial and Engineering Chemistry
• 出版年：2017
• 出版时间：25 January 2017
• 年：2017
• 卷：45
• 期：Complete
• 页码：237-240
• 全文大小：675 K
• 卷排序：45

文摘

Cell-based protein production has low throughput and restricted flexibility in process design. For the development of a rapid platform for translational analysis of genetic sequences by bypassing time- and labor-consuming steps of cell-based gene expression methods, we attempted in this study to combine the techniques of gel electrophoresis and cell-free protein synthesis in a streamlined manner. After an electrophoretic separation of the DNAs of varying sizes, DNAs in the gel matrices were directly incubated in a reaction mixture for cell-free protein synthesis, which led to successful expression of functional proteins.