Data supporting the involvement of the adenine nucleotide translocase conformation in opening the Tl⁺-induced permeability transition pore in Ca²⁺-loaded rat liver mitochondria

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• 作者：Sergey M. Korotkov¹ ; ^{korotkov@SK1645.spb.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Tl+ ; Ca2+ ; Oxygen consumption assay ; Mitochondrial swelling ; Mitochondrial membrane potential ; Rat liver mitochondria
• 刊名：Data in Brief
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：7
• 期：Complete
• 页码：620-629
• 全文大小：293 K

文摘

There we made available information about the effects of the adenine nucleotide translocase (ANT) ‘c’ conformation fixers (phenylarsine oxide (PAO), tert-butylhydroperoxide (tBHP), and carboxyatractyloside) as well as thiol reagent (4,4′-diisothiocyanostilbene-2,2′-disulfonate (DIDS)) on isolated rat liver mitochondria. We observed a decrease in A₅₄₀ (mitochondrial swelling) and respiratory control rates (RCR_ADP [state 3/state 4] and RCR_DNP [2,4-dinitrophenol-uncoupled state/basal state or state 4]), as well as an increase in Ca²⁺-induced safranin fluorescence (F_485/590, arbitrary units), showed a dissipation in the inner membrane potential (ΔΨ_mito), in experiments with energized rat liver mitochondria, injected into the buffer containing 25–75 mM TlNO₃, 125 mM KNO₃, and 100 µM Ca²⁺. The fixers and DIDS, in comparison to Ca²⁺ alone, greatly increased A₅₄₀ decline and the rate of Ca²⁺-induced ΔΨ_mito dissipation. These reagents also markedly decreased RCR_ADP and RCR_DNP. The MPTP inhibitors (ADP, cyclosporin A, bongkrekic acid, and N-ethylmaleimide) fixing the ANT in ‘m’ conformation significantly hindered the above-mentioned effects of the fixers and DIDS. A more complete scientific analysis of these findings may be obtained from the manuscript “To involvement the conformation of the adenine nucleotide translocase in opening the Tl⁺-induced permeability transition pore in Ca²⁺-loaded rat liver mitochondria” (Korotkov et al., 2016 [1]).