刊名:Biochemical and Biophysical Research Communications
出版年:2013
出版时间:27 September, 2013
年:2013
卷:439
期:3
页码:373-377
全文大小:1359 K
文摘
DNA demethylation is associated with gene activation and is mediated by a family of ten-eleven translocation (TET) dioxygenase. The TET3 protein is a 1668-amino-acid DNA demethylase that is predicted to possess five nuclear localization signals (NLSs). In this paper, we used a series of green fluorescent protein-tagged and mutation constructs to identify a conserved NLS (KKRK) embedded between amino acid 1615 and 1618 of mouse TET3. The KKRK sequence facilitates the cytoplasmic protein¡¯s translocation into the nucleus. Additionally TET3 may be imported into the nucleus by importin-¦Á and importin-¦Â.