Characterization of the nuclear localization signal of the mouse TET3 protein
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- 作者：Peng Xiao ; Xiao-long Zhou ; Hong-xiao Zhang ; Kai Xiong ; Yun Teng ; Xian-ju Huang ; Rui Cao ; Yi Wang ; Hong-lin Liu
- 关键词：TET ; ten-eleven translocation dioxygenase ; TET1 ; ten-eleven translocation dioxygenase 1 ; TET3 ; ten-eleven translocation dioxygenase 3 ; PCR ; polymerase chain reaction ; GH ; growth hormone ; GST ; glutathione S-transferase ; NLS ; nuclear localization signal ; EG
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2013
- 出版时间：27 September, 2013
- 年：2013
- 卷：439
- 期：3
- 页码：373-377
- 全文大小：1359 K
文摘
DNA demethylation is associated with gene activation and is mediated by a family of ten-eleven translocation (TET) dioxygenase. The TET3 protein is a 1668-amino-acid DNA demethylase that is predicted to possess five nuclear localization signals (NLSs). In this paper, we used a series of green fluorescent protein-tagged and mutation constructs to identify a conserved NLS (KKRK) embedded between amino acid 1615 and 1618 of mouse TET3. The KKRK sequence facilitates the cytoplasmic protein¡¯s translocation into the nucleus. Additionally TET3 may be imported into the nucleus by importin-¦Á and importin-¦Â.