CD19 controls Toll-like receptor 9 responses in human B cells

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• 作者：Henner Morbach ; MD^a ; Jean-Nicolas Schickel ; PhD^a ; Charlotte Cunningham-Rundles ; MD ; PhD^b ; Mary Ellen Conley ; MD^c ; Ismail Reisli ; MD^d ; Jose Luis Franco ; MD ; PhD^e ; Eric Meffre ; PhD^a ; ^{eric.meffre@yale.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：B cells ; Toll-like receptor 9 ; CD19 ; phosphoinositide 3-kinase ; Bruton tyrosine kinase ; AKT ; common variable immunodeficiency
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：137
• 期：3
• 页码：889-898.e6
• 全文大小：3078 K

文摘

CD19 is a B cell–specific molecule that serves as a major costimulatory molecule for amplifying B-cell receptor (BCR) responses. Biallelic CD19 gene mutations cause common variable immunodeficiency in human subjects. BCR- and Toll-like receptor (TLR) 9–induced B-cell responses are impaired in most patients with common variable immunodeficiency.

Objective

We sought to analyze whether CD19 is required for TLR9 function in human B cells.

Methods

Expression of surface activation markers was assessed after anti-IgM or CpG stimulation by using flow cytometry on B cells from patients with 1 or 2 defective CD19 alleles, which decrease or abrogate CD19 expression, respectively. The phosphorylation or interaction of signaling molecules was analyzed by using phospho flow cytometry, immunoblotting, or co-immunoprecipitation in CD19-deficient or control B cells and in a B-cell line in which CD19 has been knocked down with lentivirus-transduced short hairpin RNA.

Results

B cells from subjects with 1 or 2 defective CD19 alleles showed defective upregulation in vitro of CD86, transmembrane activator and CAML interactor (TACI), and CD23 activation markers after TLR9 stimulation. TLR9 ligands normally induce phosphorylation of CD19 through myeloid differentiation primary response gene–88 (MYD88)/proline-rich tyrosine kinase 2 (PYK2)/LYN complexes, which allows recruitment of phosphoinositide 3-kinase (PI3K) and phosphorylation of Bruton tyrosine kinase (BTK) and AKT in human B cells with a different kinetic than that of BCRs. In addition, inhibition of PI3K, AKT, or BTK, as well as BTK deficiency, also resulted in TLR9 activation defects in B cells similar to those in patients with CD19 deficiency.

Conclusion

CD19 is required for TLR9-induced B-cell activation. Hence CD19/PI3K/AKT/BTK is an essential axis integrating BCRs and TLR9 signaling in human B cells.