Multiple small versus few large amount aspirations for bone marrow harvesting in autologous and allogeneic bone marrow transplantation

详细信息    查看全文

• 作者：Volker Witt^a ; ^{volker.witt@stanna.at" class="auth_mail" title="E-mail the corresponding author} ; Herbert Pichler^a ; Gerhard Fritsch^b ; Christina Peters^a
• 关键词：Bone marrow ; Harvesting ; HPC-BM ; Stem cell transplantation
• 刊名：Transfusion and Apheresis Science
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：55
• 期：2
• 页码：221-224
• 全文大小：190 K

文摘

For successful bone marrow transplantation it is necessary to obtain enough progenitor cells during the bone marrow (BM) harvesting procedure. Most centers are using multiple aspirations of maximum 2 ml BM (A), while other centers are using few larger amount aspirations for BM harvesting (B). There is still a discussion about possible differences in graft composition between A and B. To evaluate the feasibility in children we evaluated twenty BM harvestings that were performed in 18 donors, 7 autologous (median age 6.93y; 2.48–16.6) and 13 allogeneic donors (median age 19.75y; 6.45–50.7). A and B were performed crosswise by 2 operators starting with A (2 ml) or B (100 ml) changing to B or A, collecting identically amounts with both methods. We found no statistically significant difference between A and B for MNC, T-cells, and CFU (MNC/ml 824572 versus 725000, p = 0.728; MNC/kg 3.1 10⁷ versus 2.9 10⁷, p = 0.296; CD3/ml 162500 versus 300000, p = 0.310; CFU/10⁵ MNC 1678 versus 1315, p = 0.094), but for CD34+ cells (CD34/kg 2.62 versus 2.09, p = 0.045). BM harvest by the large amount few punctures method (B) is as sufficient as the commonly used small amount frequent punctures method (A), and could be therefore used equally.