1,25-Dihydroxyvitamin D inhibits glutamine metabolism in Harvey-ras transformed MCF10A human breast epithelial cell
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- 作者：Xuanzhu Zhoua ; zhou249@purdue.edu" class="auth_mail" title="E-mail the corresponding author ; Wei Zhenga ; wzheng87@stanford.edu" class="auth_mail" title="E-mail the corresponding author ; G.A. Nagana Gowdab ; 1 ; ngowda@uw.edu" class="auth_mail" title="E-mail the corresponding author ; Daniel Rafteryb ; 2 ; draftery@uw.edu" class="auth_mail" title="E-mail the corresponding author ; Shawn S. Donkina ; sdonkin@purdue.edu" class="auth_mail" title="E-mail the corresponding author ; Brian Bequettec ; dteegard@purdue.edu" class="auth_mail" title="E-mail the corresponding author ; Dorothy Teegardena ; teegarden@purdue.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：1 ; 25(OH)2D ; 1 ; 25-dihydroxyvitamin D ; SLC1A5 ; solute carrier family 1 member 5 ; GLS ; glutaminase ; GPNA ; L-γ-glutamyl-p-nitroanilide ; VDR ; vitamin D receptor ; VDRE ; vitamin D responsive element ; NMR ; nuclear magnetic resonance ; GC&ndash ; MS ; gas chromatography&ndash ; mass spectrometry
- 刊名：Journal of Steroid Biochemistry and Molecular Biology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：163
- 期：Complete
- 页码：147-156
- 全文大小：1933 K
文摘
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Glutamine addiction is reduced by 1,25(OH)2D in MCF10A-ras cells.
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Intracellular glutamine and glutamate concentrations are reduced by 1,25(OH)2D.
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Flux of glutamine into the TCA cycle is decreased by 1,25(OH)2D.
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1,25(OH)2D inhibits glutamine uptake through the glutamine transporter SLC1A5.
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SLC1A5 is transcriptionally down-regulated through a functional VDRE.