Multinucleation and pro-inflammatory cytokine release promoted by fibrous fluoro-edenite in lung epithelial A549 cells

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• 作者：S. Travaglione ; B.M. Bruni ; L. Falzano ; P. Filippini ; A. Fabbri ; L. Paoletti and C. Fiorentini
• 关键词：Fibrous fluoro-edenite ; Crocidolite ; Epithelial cells ; Multinucleation ; Inflammatory cytokines
• 刊名：Toxicology in Vitro
• 出版年：2006
• 出版时间：September 2006
• 年：2006
• 卷：20
• 期：6
• 页码：841-850
• 全文大小：672 K

文摘

An unusual cluster of malignant mesothelioma was evidenced in Biancavilla, a Sicily village where no inhabitant had been significantly and professionally exposed to asbestos. Mineralogical and environmental studies led to the identification of a new prismatic amphibole, named fluoro-edenite. We previously reported, by using the human lung epithelial A549 cells, that prismatic fluoro-edenite was unable to induce changes that could be somehow related to cellular transformation, and this was in accordance with studies carried out in vivo. More recently, a fibrous amphibole with a composition very similar to that of prismatic fluoro-edenite, was identified in Biancavilla. This fibrous fluoro-edenite was shown to induce mesothelioma in rats. In keeping with this effect in vivo, in the present work we observed multinucleation and spreading, common features of transformed cells, as well as pro-inflammatory cytokine release in A549 cells. Such cell changes occurred without interfering with the passage of the resulting multinucleated cells through the cell cycle and without condemning cells to death. Hence, in lung epithelial cells, fibrous fluoro-edenite behaved similarly to the unrelated asbestos type crocidolite, whose connection with severe inflammation and cancer of the lung is renowned.