Rho-activating Escherichia coli cytotoxic necrotizing factor 1: macropinocytosis of apoptotic bodies in human epithelial cells

详细信息    查看全文

• 作者：Alessia Fabbri ; Loredana Falzano ; Sara Travaglione ; Annarita Stringaro ; Walter Malorni ; Stefano Fais ; Carla Fiorentini
• 关键词：macropinocytosis ; CNF1 ; apoptotic cells ; Rho GTPases
• 刊名：International Journal of Medical Microbiology
• 出版年：2001
• 出版时间：2001
• 年：2001
• 卷：291
• 期：6-7
• 页码：551-554
• 全文大小：763 K

文摘

Some pathogenic Escherichia coli strains produce a protein toxin, named cytotoxic necrotizing factor 1 (CNF1), which permanently activates proteins belonging to the Rho family. In epithelial cells, the consequence of this activation is the rearrangement of the actin cytoskeleton and the promotion of an intense and generalized ruffling activity. This leads, in turn, to the induction of a phagocytic-like behavior called macropinocytosis that, in the case of CNF1, depends on the coordinate activation of Rho, Rac and Cdc42. Following internalization, the ingested material is discharged into Rab-7 and Lamp-1-positive acidic vesicles where it probably undergoes degradation. By exerting this activity, CNF1-activated epithelial cells might support the scavenging activity of macrophages during bacterial overgrowth.