Label-free, arrayed sensing of immune response to influenza antigens
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- 作者：Charles R. Mace ; David J. Topham ; Tim R. Mosmann ; Sally A. Quataert ; John J. Treanor ; Benjamin L. Miller
- 关键词：Biosensor ; Microarray ; Influenza
- 刊名：Talanta
- 出版年：2011
- 出版时间：15 January 2011
- 年：2011
- 卷：83
- 期：3
- 页码：1000-1005
- 全文大小：630 K
文摘
Periodic outbreaks of pandemic influenza have been a devastating cause of human mortality over the past century. More recently, an avian influenza strain, designated H5N1, has been identified as having the potential to cause a zoogenic pandemic in humans, and a current outbreak of a new H1N1 influenza variant hypothesized to be of swine origin is of considerable concern. In order to facilitate surveillance and the rapid assessment and comparison of vaccination efforts, a high-throughput assay is highly desirable to supplement standard methods, which require high biosafety-level facilities. In this paper, we describe the design, production, and preliminary evaluation of an antigen array incorporating a panel of hemagglutinins as a platform for the detection and rapid quantification of influenza-specific antibodies in human serum by Arrayed Imaging Reflectometry (AIR), a label-free optical biosensor.