TRESK potassium channel in human T lymphoblasts

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• 作者：Dé ; nison Selene Sá ; nchez-Miguel^a ; ¹ ; ^{amurusk@hotmail.com} ; Fernando Garcí ; a-Dolores^b ; ^{garciaddf@yahoo.com} ; Marí ; a Rosa Flores-Má ; rquez^c ; ^{mariafo31@yahoo.com.mx} ; Ivá ; n Delgado-Enciso^d ; Igor Pottosin^a ; ^{pottosin@ucol.mx} ; Oxana Dobrovinskaya^a ; ^{oxana@ucol.mx}
• 关键词：TRESK ; KCNK18 ; T cell ; Jurkat ; Leukemia ; Lymphoma
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2013
• 出版时间：3 May, 2013
• 年：2013
• 卷：434
• 期：2
• 页码：273-279
• 全文大小：2919 K

文摘

TRESK (TWIK-related spinal cord K⁺) channel, encoded by KCNK18 gene, belongs to the double-pore domain K⁺ channel family and in normal conditions is expressed predominantly in the central nervous system. In our previous patch-clamp study on Jurkat T lymphoblasts we have characterized highly selective K⁺ channel with pharmacological profile identical to TRESK. In the present work, the presence of KCNK18 mRNA was confirmed in T lymphoblastic cell lines (Jurkat, JCaM, H9) but not in resting peripheral blood lymphocytes of healthy donors. Positive immunostaining for TRESK was demonstrated in lymphoblastic cell lines, in germinal centers of non-tumoral lymph nodes, and in clinical samples of T acute lymphoblastic leukemias/lymphomas. Besides detection in the plasma membrane, intracellular TRESK localization was also revealed. Possible involvement of TRESK channel in lymphocyte proliferation and tumorigenesis is discussed.