This is the first report on RB5 biodegradation by Trichoderma atroviride.
Laccase played a major role in catalysing the biodegradation of RB5.
2,4-ditertbutylphenol, 1,2,4-trimethylbenzene and benzoic acid-TMS are degradation products.
RB5 biodegradation by T. atroviride does not generate toxic aromatic amines.