An artificial transcription activator xyr1-cre1b was expressed in T. reesei Rut C30.
The mutants displayed constitutive (hemi) cellulase production from glucose.
FPase of 2.63 IU/mL was achieved within short time (48 h) in a 7-L fermenter.
The crude enzyme is suitable for sugar release from alkali pretreated corn stover.