Wintergreen oil: A novel method in Wheatley's trichrome staining technique

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• 作者：Fatmah Md Salleh ; Tengku Shahrul Anuar ; Azlin Mohd Yasin ; Norhayati Moktar
• 关键词：Wintergreen oil ; Wheatley's trichrome ; Staining technique
• 刊名：Journal of Microbiological Methods
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：91
• 期：1
• 页码：174-178
• 全文大小：1305 K

文摘

Permanent staining of faecal smears by Wheatley's trichrome technique has been used by many scientists for the detection of parasites in the past and it was found to be highly sensitive. This study was conducted to evaluate the use of Wintergreen oil in comparison with xylene in Wheatley's trichrome staining technique, as the reference technique. In a blind comparison study, 500 collected faecal samples from aboriginal communities were examined. Wintergreen oil was found to be more superior than xylene as a clearing agent in the Wheatley's trichrome staining of polyvinyl alcohol-fixed faecal smears for the identification of intestinal protozoa. Elimination of toxic, carcinogenic, and fire hazards makes Wintergreen oil the preferred choice in routine parasitology examinations.