Diallyl trisulfide suppresses dextran sodium sulfate-induced mouse colitis: NF-¦ÊB and STAT3 as potential targets

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• 作者：Hyung-Jun Lee ; Hee Geum Lee ; Ki-Seok Choi ; Young-Joon Surh ; Hye-Kyung Na
• 关键词：Diallyl trisulfide ; NF-¦ÊB ; STAT3 ; Mouse colitis
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2013
• 出版时间：26 July, 2013
• 年：2013
• 卷：437
• 期：2
• 页码：267-273
• 全文大小：1432 K

文摘

Diallyl trisulfide (DATS), one of the volatile constituents of garlic oil, has been reported to possess antioxidant, anti-inflammatory, and anti-carcinogenic properties. In this study, DATS (10 ¦Ìmol) given orally for 7 days before and for another 7 days after starting administration of 2.5 % dextran sulfate sodium (DSS) in drinking water protected against colitis induced by DSS in male ICR mice. DATS significantly inhibited the DSS-induced DNA binding of NF-¦ÊB, phosphorylation of I¦ÊB¦Á and the expression of pro-inflammatory proteins, such as cyclooxygenase-2 and inducible nitric oxide synthase, which are major target proteins of NF-¦ÊB. The DSS-induced DNA binding and phosphorylation at the Tyr 705 residue of signal transducer and activator of transcription 3 (STAT3), and expression of its major target protein cyclin D1 in mouse colonic mucosa were also attenuated by DATS administration. Likewise, DSS-induced phosphorylation of extracellular signal-regulated kinase 1/2 was suppressed by DATS treatment. In conclusion, DATS ameliorates the DSS-induced mouse colitis presumably by blocking inflammatory signaling mediated by NF-¦ÊB and STAT3.