An embryonic transcriptome of the pulmonate snail Radix balthica

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• 作者：Oliver Tills ; ^{oliver.tills@plymouth.ac.uk" class="auth_mail" title="E-mail the corresponding author} ; Manuela Truebano ^{manuela.truebanogarcia@plymouth.ac.uk" class="auth_mail" title="E-mail the corresponding author} ; Simon Rundle ^{s.rundle@plymouth.ac.uk" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Transcriptome ; Gastropod ; Radix balthica ; Predator cue
• 刊名：Marine Genomics
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：24
• 期：part_P3
• 页码：259-260
• 全文大小：172 K

文摘

The pond snail, m>Radix balthicam> (Linnaeus 1758), is an emerging model species within ecological developmental biology. While its development has been characterised in detail, genomic resources for embryonic stages are lacking. We applied Illumina MiSeq RNA-seq to RNA isolated from pools of embryos at two points during development. Embryos were cultured in either the presence or absence of predator kariomones to increase the diversity of the transcripts assembled. Sequencing produced 47.2 M paired-end reads, assembled into 54,360 contigs of which 73% were successfully annotated. This transcriptome provides an invaluable resource to build a mechanistic understanding of developmental plasticity.