Antinociceptive activity of Tilia americana

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• 作者：Ana Laura Martí ; nez ; Ma. Eva Gonzá ; lez-Trujano ; Eva Aguirre-Herná ; ndez ; Julia Moreno ; Marco Soto-Herná ; ndez ; Francisco J. Ló ; pez-Muñ ; oz
• 关键词：Antinociception ; Flavonoids ; HPLC ; Quercetin ; Tilia americana var. mexicana ; 5-HT_1A receptors
• 刊名：Neuropharmacology
• 出版年：2009
• 出版时间：February 2009
• 年：2009
• 卷：56
• 期：2
• 页码：564-571
• 全文大小：411 K

文摘

Tilia species are well known around the world for their properties in traditional medicine. Antinociceptive activity of hexane, methanol and aqueous extracts from Tilia americana var. mexicana inflorescences was evaluated in the pain-induced functional impairment model in rats (PIFIR). A preliminar 300 mg/kg dosage of aqueous extracts i.p., but not the same dose of methanol or hexane extract, produced an antinociceptive response in rats similar to that of tramadol (17.8 mg/kg i.p.). A dose–response curve from aqueous extract allowed the determination of ED₅₀ = 364.97 mg/kg in comparison to ED₅₀ = 10.35 mg/kg for tramadol in this model. A previous HPLC–DAD analysis corroborated by an HPLC–MS technique in this study demonstrated the flavonoid composition in this Tilia aqueous extract revealing the presence of glycosides mainly derived from quercetin. Thus, Tilia aqueous extract and quercetin were tested at 30 and/or 100 mg/kg dosages i.p. in the PIFIR and formalin models producing a significant and dose-dependent antinociceptive response resembling that produced by a total and a partial agonist of 5-HT_1A receptors like 8-OH-DPAT (0.1 mg/kg, s.c.) and buspirone (5 mg/kg, i.p.), respectively. In all the treatments, antinociceptive response was inhibited in the presence of WAY 100635 (0.12 mg/kg, i.p.). Our results support the analgesic activity of T. americana var. mexicana inflorescences attributed by folk medicine; they also indicate that quercetin is partly responsible for this pharmacological activity that is likely mediated by serotonin 5-HT_1A receptors.