Release of the cytosolic tryparedoxin peroxidase into the incubation medium and a different profile of cytosolic and mitochondrial peroxiredoxin expression in H₂O₂-treated Trypanosoma cruzi tissue culture-derived trypomastigotes

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• 作者：F.R. Gadelha^a ; C.C. Gonç ; alves^a ; E.C. Mattos^b ; M.J.M. Alves^b ; M.D. Piñ ; eyro^c ; C. Robello^c ; E.F. Peloso^a ; ^{eduardofpeloso@yahoo.com.br}
• 关键词：Trypanosoma cruzi ; Oxidative stress ; Tryparedoxin peroxidases
• 刊名：Experimental Parasitology
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：133
• 期：3
• 页码：287-293
• 全文大小：537 K

文摘

Trypanosoma cruzi antioxidant enzymes are among the factors that guarantee parasite survival and maintain infection, enabling T. cruzi to cope with oxidative stress. Herein, the expression of cytosolic (TcCPx) and mitochondrial (TcMPx) tryparedoxin peroxidases was evaluated in tissue culture-derived trypomastigotes upon incubation with different concentrations of H₂O₂. TcCPx expression slightly increased (5.4 % ) in cells submitted to 10 ¦ÌM H₂O₂ treatment when compared to the control, but decreased when higher H₂O₂ concentrations (20-50 ¦ÌM) were employed. Under these conditions, TcMPx expression increased (¡«53 % ) with 10 ¦ÌM-treatment compared to the control, followed by a reduction that reached ¡«46 % of the control when using the highest concentration tested. Interestingly, in the supernatant of the incubations, TcCPx, but not TcMPx, was detected, and its levels increased concomitantly with its decreased expression in the intracellular compartment. Our data show that peroxiredoxins in the tissue culture-derived trypomastigote can be modulated under oxidative stress and are present in higher amounts when compared to the epimastigote stage of T. cruzi. Additionally, due to the different expression patterns observed upon H₂O₂-treatment, each peroxiredoxin may play a distinct role in protecting the parasite under oxidative stress conditions.