Redox metabolism in Trypanosoma cruzi: Functional characterization of tryparedoxins revisited

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• 作者：Diego G. Arias ; Vanina E. Marquez ; Mar¨ªa L. Chiribao ; Fern ; a R. Gadelha ; Carlos Robello ; Alberto A. Iglesias ; Sergio A. Guerrero
• 关键词：Grx ; glutaredoxin ; GR ; glutathione reductase ; TR ; trypanothione reductase ; T(SH)2 ; trypanothione ; TS2 ; trypanothione disulfide ; GSSG ; glutathione disulfide ; GSH ; reduced glutathione ; TXNPx ; 2-Cys-peroxiredoxin-type tryparedoxin peroxidase ; GPxI ; glutathio
• 刊名：Free Radical Biology and Medicine
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：63
• 期：Complete
• 页码：65-77
• 全文大小：2390 K

文摘

Tryparedoxins (TXNs) are multipurpose oxidoreductases from trypanosomatids that transfer reducing equivalents from trypanothione to various thiol proteins. In Trypanosoma cruzi, two genes coding for TXN-like proteins have been identified: TXNI, previously characterized as a cytoplasmic protein, and TXNII, a putative tail-anchored membrane protein. In this work, we performed a comparative functional characterization of T. cruzi TXNs. Particularly, we cloned the gene region coding for the soluble version of TXNII for its heterologous expression. The truncated recombinant protein (without its 22 C-terminal transmembrane amino acids) showed TXN activity. It was also able to transfer reducing equivalents from trypanothione, glutathione, or dihydrolipoamide to various acceptors, including methionine sulfoxide reductases and peroxiredoxins. The results support the occurrence and functionality of a second tryparedoxin, which appears as a new component in the redox scenario for T. cruzi.