Analysis of interferon gamma protein expression in zebrafish (Danio rerio)
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- 作者：Sohye Yoona ; sohyeyoon@abdn.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Ayham Alnabulsib ; Ting Yu Wanga ; 1 ; Po Tsang Leea ; 2 ; Tzong-Yueh Chenc ; Steve Birda ; d ; Jun Zoua ; Christopher J. Secombesa ; c.secombes@abdn.ac.uk" class="auth_mail" title="E-mail the corresponding author
- 关键词：Zebrafish ; IFN-γ ; Monoclonal antibody ; Protein expression ; PHA ; Antigen re-stimulation
- 刊名：Fish & Shellfish Immunology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：57
- 期：Complete
- 页码：79-86
- 全文大小：1009 K
文摘
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A monoclonal antibody to zebrafish IFN-γ has been produced and characterised.
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The moAb detected induced IFN-γ protein expression following PHA stimulation of kidney/spleen leukocytes.
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Antigen specific induction of IFN-γ protein in zebrafish following immunization and re-stimulation was also demonstrated.
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A positive correlation between mRNA and protein levels of IFN-γ in zebrafish was revealed in this study.