Downgrading the systemic condition of rabbits after long term exposure to cypermethrin and piperonyl butoxide

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• 作者：Alexander I. Vardavas^a ; Persefoni Fragkiadaki^a ; Athanasios K. Alegakis^a ; Dimitrios Kouretas^b ; Nikolaos Goutzourelas^b ; John Tsiaoussis^c ; Christina Tsitsimpikou^d ; Polychronis D. Stivaktakis^a ; Fé ; lix Carvalho^e ; Aristidis M. Tsatsakis^a ; ^{aris@med.uoc.gr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Oxidative stress ; Telomerase activity ; Synergistic action ; Cypermethrin ; Piperonyl butoxide
• 刊名：Life Sciences
• 出版年：2016
• 出版时间：15 January 2016
• 年：2016
• 卷：145
• 期：Complete
• 页码：114-120
• 全文大小：376 K

文摘

The aim of this study is to clarify the effect of cypermethrin (CY) on the oxidative stress (OS) and inflammation status of animals exposed to it and the synergistic role of piperonyl butoxide (PB0).

Main methods

Markers of oxidative stress, such as total antioxidant activity (TAC), protein carbonyls, hemoglobin (Hb), reduced glutathione (GSH), thiobarbituric-acid reactive substances (TBARS), along with the telomerase activity in PBMCs (peripheral blood mononuclear cells) were analyzed.

Key findings

Oxidative stress markers showed statistically significant differences between groups in TAC (p < 0.001), GSH (p = 0.018) and CAT activity (p = 0.029), which depended on dose and combined effect of both compounds. Telomerase activity also showed a statistically significant difference between all groups (F = 43.48, df = 6, 14, p < 0.001) with cypermethrin, piperonyl butoxide and the co-exposed groups being significantly different from the control group (p < 0.05). Significance: The observed results for TBARS, GSH, Hb, TAC, Crbnls and CAT from our exposed groups showed altered levels compared to control groups that could be linked to doses and combined effects of each chemical substance (cypermethrin and piperonyl butoxide). Oxidative stress markers suggest that cypermethrin, piperonyl butoxide and the co-exposed groups, induce oxidative stress as well as induction of telomerase activity.