Characterization of monoclonal antibodies directed against the canine distemper virus nucleocapsid protein
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- 作者：Munemitsu Masuda ; Hiroki Sato ; Hiroshi Kamata ; Tomoe Katsuo ; Akiko Takenaka ; Ryuichi Miura ; Misako Yoneda ; Kyoko Tsukiyama-Kohara ; Kiyohisa Mizumoto and Chieko Kai
- 关键词：Canine distemper virus ; Nucleocapsid protein ; Monoclonal antibodies ; Deletion mutants ; Antigenic domain ; Competitive ELISA
- 刊名：Comparative Immunology, Microbiology and Infectious Diseases
- 出版年：2006
- 出版时间：March-May 2006
- 年：2006
- 卷：29
- 期：2-3
- 页码：157-165
- 全文大小：136 K
文摘
We have established four monoclonal antibodies (MAbs) against the nucleocapsid protein (NP) of canine distemper virus (CDV). A competitive binding assay has revealed that the MAbs are directed against two antigenic domains. An immunofluorescence assay using a series of deletion clones of the NP and an immunoprecipitation assay using the NP have revealed that two of the MAbs recognize the C-terminal region of the NP while the other two recognize the tertiary structure of the N-terminal domain. These MAbs reacted with all eight strains of CDV used in this study, but showed different reactivities against measles virus and rinderpest virus.