Catalase encapsulated in mesoporous silica and its performance

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• 作者：Tetsuji Itoh ; Ryo Ishii ; Shun-ichi Matsuura ; Satoshi Hamakawa ; Takaaki Hanaoka ; Tatsuo Tsunoda ; Junko Mizuguchi ; Fujio Mizukami
• 关键词：Catalase ; Mesoporous silica
• 刊名：Biochemical Engineering Journal
• 出版年：2009
• 出版时间：15 May 2009
• 年：2009
• 卷：44
• 期：2-3
• 页码：167-173
• 全文大小：898 K

文摘

Catalase was confined in a nanospace of about 7 nm in diameter in mesoporous silica (FSM; folded-sheet mesoporous material), forming a catalase-FSM conjugate. That the enzyme was encapsulated in the pores of mesoporous materials was proven by the direct visualization technique. The catalase-FSM showed not only an activity similar to that of the native catalase in the decomposition of H₂O₂ but also much higher stability: specifically, the catalase immobilized in FSM still retained its original activity after being applied 50 times to the decomposition, whereas the native lost its activity after 20 times recycling. Furthermore, the catalase-FSM showed catalase activity even in an organic media in which the native catalase had almost no catalytic activity.