Synthesis of l-theanine using enzyme/mesoporous silica conjugates under high pH conditions

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• 作者：Takuji Yokoyama ; Ryo Ishii ; Tetsuji Itoh ; Koichi Kitahata ; Shun-ichi Matsuura ; Tatsuo Tsunoda ; Satoshi Hamakawa ; Taka-aki Hanaoka ; Hironobu Nanbu ; Fujio Mizukami
• 关键词：Enzyme immobilization ; Mesoporous silica ; Zirconia incorporation ; l-Theanine
• 刊名：Materials Letters
• 出版年：2011
• 出版时间：15 January 2011
• 年：2011
• 卷：65
• 期：1
• 页码：67-69
• 全文大小：503 K

文摘

We present a new enzymatic process for synthesis of l-theanine using glutaminase combined with immobilization technique on a mesoporous silica (MPS). The MPS was firstly attempted to modify with zirconia in order to enhance the durability against the reaction under high pH conditions. The glutaminase on the MPS successfully catalyzed the reaction for the synthesis of l-theanine. The glutaminase/MPS conjugate was subsequently recovered and employed for the reaction again. The conjugate showed the corresponding activity to the first synthesis. This indicates that the conjugate functions as a catalyst for synthesis of l-theanine, having the operational stability sufficient for reuse.