Protein aggregation under high concentration/density state during chromatographic and ultrafiltration processes

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• 作者：Tsutomu Arakawa^a ; ^{tarakawa@ap-lab.com} ; Daisuke Ejima^b ; Teruo Akuta^c
• 关键词：High concentration ; High density ; Aggregation ; Chromatography ; Ultrafiltration
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：1153-1158
• 全文大小：727 K
• 卷排序：95

文摘

Local transient high protein concentration or high density condition can occur during processing of protein solutions. Typical examples are saturated binding of proteins during column chromatography and high protein concentration on the semi-permeable membrane during ultrafiltration. Both column chromatography and ultrafiltration are fundamental technologies, specially for production of pharmaceutical proteins. We summarize here our experiences related to such high concentration conditions.