• 作者：Hanae Takatsuki ; PhD^a ; Takayuki Fuse ; PhD^a ; Takehiro Nakagaki ; MD ; PhD^a ; Tsuyoshi Mori ; PhD^b ; Ban Mihara ; MD ; PhD^c ; Masaki Takao ; MD ; PhD^c ; ^d ; Yasushi Iwasaki ; MD ; PhD^e ; Mari Yoshida ; MD ; PhD^e ; Shigeo Murayama ; MD ; PhD^f ; Ryuichiro Atarashi ; MD ; PhD^b ; Noriyuki Nishida ; MD ; PhD^a ; Katsuya Satoh ; MD ; PhD^g ; ^{satoh-prion@nagasaki-u.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Prion ; Prion-seeding activity ; SD50 ; Non-neural tissue ; Creutzfeldt-Jakob disease
• 刊名：EBioMedicine
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：12
• 期：Complete
• 页码：150-155
• 全文大小：799 K

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Prion-seeding activities reach 10⁶/g from a 50% seeding dose in non-neuronal tissues.

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Results suggest that prion-seeding activity exists in neural and non-neural organs.

A major problem for the diagnosis and management of human prion diseases is the lack of rapid and high-sensitive assays to measure low prion levels. Recent studies have tried to measure prion concentrations in non-neuronal tissues, but prion levels were not sufficient. Therefore, we developed the RT-QuIC method to measure prion-seeding activity in the non-neuronal, human tissues. The SD50 levels in the spleen, kidney, lung, and liver were 5.0–6.5, with different SD50 levels in the individual cases.