Proteomics analysis of the endogenous, constitutive, leaf SUMOylome
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- 作者：Bertrand Colignona ; b ; 1 ; Edouard Delaiveb ; Marc Dieub ; c ; Catherine Demazyb ; c ; Yordan Muhovskia ; Cindy Wallona ; Martine Raesb ; Sergio Mauroa ; mauro@cra.wallonie.be" class="auth_mail" title="E-mail the corresponding author
- 关键词：3D gel electrophoresis ; Post translational modification ; SUMOylation ; Endogenous SUMO conjugates ; Solanum tuberosum
- 刊名：Journal of Proteomics
- 出版年：2017
- 出版时间：6 January 2017
- 年：2017
- 卷：150
- 期：Complete
- 页码：268-280
- 全文大小：1313 K
文摘
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The paper reports the development of a new application for proteomic quantitative analysis using 3D DIGE.
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Using the new method, endogenous SUMO protein conjugates were identified in a complex organ, the developing potato leaf.
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30 of these 39 identified proteins were not previously known to be SUMO targets.
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The method offers a quantitative analysis of targets for SUMOylation, whether singly or in interaction with other PTMs.