Gamma-tubulin coordinates nuclear envelope assembly around chromatin
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- 作者：Catalina Ana Rosselló ; Lisa Lindströ ; m ; Johan Glindre ; Greta Eklund ; Maria Alvarado-Kristensson ; maria.alvarado-kristensson@med.lu.se" class="auth_mail" title="E-mail the corresponding author
- 关键词：Biological sciences ; Cell biology
- 刊名：Heliyon
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：2
- 期：9
- 全文大小：6525 K
文摘
The cytosolic role of γ-tubulin as a microtubule organizer has been studied thoroughly, but its nuclear function is poorly understood. Here, we show that γ-tubulin is located throughout the chromatin of demembranated Xenopus laevis sperm and, as the nucleus is formed, γ-tubulin recruits lamin B3 and nuclear membranes. Immunodepletion of γ-tubulin impairs X. laevis assembly of both the lamina and the nuclear membrane. During nuclear formation in mammalian cell lines, γ-tubulin establishes a cellular protein boundary around chromatin that coordinates nuclear assembly of the daughter nuclei. Furthermore, expression of a γ-tubulin mutant that lacks the DNA-binding domain forms chromatin-empty nuclear like structures and demonstrate that a constant interplay between the chromatin-associated and the cytosolic pools of γ-tubulin is required and, when the balance between pools is impaired, aberrant nuclei are formed. We therefore propose that the nuclear protein meshwork formed by γ-tubulin around chromatin coordinates nuclear formation in eukaryotic cells.