Increased levels of serum granzyme-B is associated with insulin resistance and increased cardiovascular risk in adolescent polycystic ovary syndrome patients

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• 作者：Efser Oztas^a ; ^{efseroztas@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Sibel Ozler^a ; Aytekin Tokmak^a ; Nafiye Yilmaz^a ; Huseyin Tugrul Celik^b ; Fatmanur Hacıevliyagil Kazancı^b ; Nuri Danisman^a ; Merve Ergin^c ; Halil Ibrahim Yakut^d
• 关键词：Adolescent polycystic ovary syndrome ; Cardiovascular risk ; Granzyme-B ; Insulin resistance ; Perforin
• 刊名：European Journal of Obstetrics & Gynecology and Reproductive Biology
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：198
• 期：Complete
• 页码：89-93
• 全文大小：311 K

文摘

Our aim was to determine serum perforin and granzyme-B levels in adolescent PCOS patients, and to investigate whether they are associated with some of the insulin sensitivity, obesity and cardiovascular (CV) risk markers and metabolic syndrome.

Study design

A case–control study was carried out including a total of 172 adolescents (83 PCOS patients and 89 age-matched healthy controls). Participants were recruited consecutively. Homeostasis model assessment (HOMA-IR), lipid parameters, and anthropometric measurements were determined. Serum perforin and granzyme B levels were measured by commercially available ELISA kits. HOMA-IR > 3.16 was considered to indicate the presence of insulin resistance. Logistic regression analysis was applied for the predictive value of granzyme-B for increased CV risk in PCOS patients.

Results

As body mass index (BMI) of the PCOS patients was significantly higher than the controls (median 24.6 kg/m² and 21.4 kg/m², respectively, p < 0.001) all parameters were evaluated after adjustment for BMI. Adolescents with PCOS had significantly higher levels of fasting glucose, insulin, HOMA-IR and granzyme-B when compared with controls. According to the results of logistic regression analysis, granzyme-B levels were found to be significantly associated with increased HOMA-IR (OR = 6.120, 95% CI: 2.352–15.926, p < 0.001) in adolescent PCOS patients. Additionally, elevated levels of serum granzyme-B were predictive for increased CV risk in PCOS patients (OR = 0.237, 95% CI: 0.091–0.616, p = 0.003).

Conclusions

Increased levels of serum granzyme-B are independently associated with insulin resistance and also with increased CV risk in adolescent polycystic ovary syndrome patients.